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Fig. 1 | BMC Research Notes

Fig. 1

From: Attempted use of PACE for riboswitch discovery generates three new translational theophylline riboswitch side products

Fig. 1

PACE experimental design for the discovery of new RSs. a During positive selection, anhydrotetracycline (aTc) concentration is reduced while the new target ligand xanthine concentration remained constant. Mutagenesis cassette is induced using arabinose. The chemostat replenishes growth media and uninfected E. coli. b Negative selection employed a dominant gIII negative allele (gIII neg) induced by a steady concentration of theophylline to select against theophylline-specific riboswitches while gradually reducing aTc to maintain phage with xanthine-specific riboswitches

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