Fig. 2
From: Validation of extracellular ligand–receptor interactions by Flow-TriCEPS
Flow-TriCEPS knock down target validation of a protein (TRFE) and an antibody (anti EGFR) on MDA-MB-231 cells. a Flow cytometric analysis comparing binding of anti EGFR-PE labelled antibody on MDA-MB-231 cells transfected with scrambled or EGFR siRNA (Hs_EGFR_10) for 72 h. Igg-PE was used as control. A representative experiment out of two is shown. b Flow cytometric analysis comparing binding of EGFR Ab-TriCEPS on MDA-MB-231 cells transfected with scrambled or EGFR siRNA (Hs_EGFR_10) for 72 h. Gly-TriCEPS and cells labelled with PE-streptavidin were used as control. A representative experiment out of two is shown