Fig. 3
From: Preparation of lyophilized recombinant prion protein for TSE diagnosis by RT-QuIC
Comparison of seeding activity of RT-QuIC reactions using a–c resolubilized after lyophilization and d–f freshly prepared BV rPrP as substrates. RT-QuIC reactions were seeded with 10−3 (blue), 10−4 (red), and 10−5 (green) dilutions of two scrapie-infected sheep brains from 2 different scrapie positive sheep a and d are the same infected sheep, b and e are the same infected sheep and a negative control (uninfected sheep, c and f) brain homogenates with the addition of 0.001% of SDS. Shown are the average ThT fluorescence readings (thick lines) with standard deviations (thin lines) determined from all replicates (four replicate reactions per brain dilution). The results of a, b, d and e all meet the criteria to be determined positive by RT-QuIC as defined in the methods