Fig. 1

Identification and validation of genes that regulate GBP/PLC-mediated Ca²⁺ signaling. a Representative Ca²⁺-signaling responses from Drosophila S3 cells in plate 116B88 that were pre-treated with either control dsRNAi (traces ‘a’ and ‘c’) or our dsRNAi construct against Itp-r83A (trace ‘b’) before addition of either vehicle (trace ‘a’) or 500 nM GBP (traces ‘b’ and ‘c’). b reproducibility of dsRNA replicates obtained from 23 plates assayed in duplicate. c Z-scores from all amplicons. d Effects upon [Ca²⁺]_T after treatment of cells with our dsRNA constructs against the indicated genes; n = 4; *p < 0.001