Fig. 1

Relative expression rate of the virA, virD3, and virG genes in bacterial cells of the Rhizobium vitis Ti strain in grapevine plants measured using the reverse transcriptase quantitative polymerase chain reaction (RT-qPCR). Values (%) represent the relative expression rate of the vir genes at 1 day after inoculation (dai) compared with the value when only Ti strain VAT03-9 was inoculated (“Ti strain alone”), which had a value of 100%. Each cell suspension or mixture (ARK-1 plus a Ti, VAR06-30 plus a Ti, and a Ti alone) at cell ratios of 1:1 was inoculated onto the stems of grapevine plants after wounding. Stem samples were harvested at 1 dai. The center bar of the boxplot is the median, the lower and upper horizontal bars are 25th and 75th percentile, and whiskers show 95% range. Boxes labeled with different letters indicate a significant difference from the other bars in same gene expression (P ≤ 0.05, Tukey’s HSD test)