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Table 1 Oligonucleotide primers and probes used in the RT-qPCR analyses

From: Biological control agent Rhizobium (=Agrobacterium) vitis strain ARK-1 suppresses expression of the essential and non-essential vir genes of tumorigenic R. vitis

Name Primer or probe 5′-3′ nucleotide sequence Target DNA Reference Accession no. Design or supplier
virA-F Primer CTC GAC TGA AAG AAG GAC ATA CTC A virA This study CP000637 Licensed Dual-Labeled Probes for qPCR (Sigma-Aldrich)
virA-R Primer CGC AAG AAG GTC TAT CAT AAG GAA G virA This study CP000637 Licensed Dual-Labeled Probes for qPCR (Sigma-Aldrich)
virA-P Probe AAT GGT TCC GCT CGC AAG CAC CTT G virA This study CP000637 Licensed Dual-Labeled Probes for qPCR (Sigma-Aldrich)
virD3-F1P Primer GTC AAC TCG GGG AAG TCG T virD3 This study CP000637 ProbeFinder (Roche Diagnostics)
virD3-R1P Primer CAG TTC AGT TCG ATC GCT TG virD3 This study CP000637 ProbeFinder (Roche Diagnostics)
virD3-P Probe TTC CTC TG virD3 This study CP000637 Universal ProbeLibrary probe #6 Cat. no. 04685032001 (Roche Diagnostics)
virG-F Primer CTG TCT CGG ATG CGA GTA CG virG This study CP000637 Licensed Dual-Labeled Probes for qPCR (Sigma-Aldrich)
virG-R Primer CGG AGT ATC TAA CGA TCC ACG C virG This study CP000637 Licensed Dual-Labeled Probes for qPCR (Sigma-Aldrich)
virG-P Probe TGA CCG CCG TAT CCG ATA GTC AGC AAT T virG This study CP000637 Licensed Dual-Labeled Probes for qPCR (Sigma-Aldrich)
VAT03-9pyrG-F-2 Primer GGT GAA GTC TTC GTC ACC GAC pyrG [14] AB272142 Licensed Dual-Labeled Probes for qPCR (Sigma-Aldrich)
VAT03-9pyrG-R-2 Primer CGC CCT GTG AAG CGT TCA TAG pyrG [14] AB272142 Licensed Dual-Labeled Probes for qPCR (Sigma-Aldrich)
VAT03-9pyrG-P-2 Probe CGG CGC AGA GAC CGA CCT TGA TCT TG pyrG [14] AB272142 Licensed Dual-Labeled Probes for qPCR (Sigma-Aldrich)