Table 1 Oligonucleotide primers and probes used in the RT-qPCR analyses
Name | Primer or probe | 5′-3′ nucleotide sequence | Target DNA | Reference | Accession no. | Design or supplier |
|---|---|---|---|---|---|---|
virA-F | Primer | CTC GAC TGA AAG AAG GAC ATA CTC A | virA | This study | CP000637 | Licensed Dual-Labeled Probes for qPCR (Sigma-Aldrich) |
virA-R | Primer | CGC AAG AAG GTC TAT CAT AAG GAA G | virA | This study | CP000637 | Licensed Dual-Labeled Probes for qPCR (Sigma-Aldrich) |
virA-P | Probe | AAT GGT TCC GCT CGC AAG CAC CTT G | virA | This study | CP000637 | Licensed Dual-Labeled Probes for qPCR (Sigma-Aldrich) |
virD3-F1P | Primer | GTC AAC TCG GGG AAG TCG T | virD3 | This study | CP000637 | ProbeFinder (Roche Diagnostics) |
virD3-R1P | Primer | CAG TTC AGT TCG ATC GCT TG | virD3 | This study | CP000637 | ProbeFinder (Roche Diagnostics) |
virD3-P | Probe | TTC CTC TG | virD3 | This study | CP000637 | Universal ProbeLibrary probe #6 Cat. no. 04685032001 (Roche Diagnostics) |
virG-F | Primer | CTG TCT CGG ATG CGA GTA CG | virG | This study | CP000637 | Licensed Dual-Labeled Probes for qPCR (Sigma-Aldrich) |
virG-R | Primer | CGG AGT ATC TAA CGA TCC ACG C | virG | This study | CP000637 | Licensed Dual-Labeled Probes for qPCR (Sigma-Aldrich) |
virG-P | Probe | TGA CCG CCG TAT CCG ATA GTC AGC AAT T | virG | This study | CP000637 | Licensed Dual-Labeled Probes for qPCR (Sigma-Aldrich) |
VAT03-9pyrG-F-2 | Primer | GGT GAA GTC TTC GTC ACC GAC | pyrG | [14] | AB272142 | Licensed Dual-Labeled Probes for qPCR (Sigma-Aldrich) |
VAT03-9pyrG-R-2 | Primer | CGC CCT GTG AAG CGT TCA TAG | pyrG | [14] | AB272142 | Licensed Dual-Labeled Probes for qPCR (Sigma-Aldrich) |
VAT03-9pyrG-P-2 | Probe | CGG CGC AGA GAC CGA CCT TGA TCT TG | pyrG | [14] | AB272142 | Licensed Dual-Labeled Probes for qPCR (Sigma-Aldrich) |