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Fig. 2 | BMC Research Notes

Fig. 2

From: Development of a new method for collecting hemolymph and measuring phenoloxidase activity in Tribolium castaneum

Fig. 2

Dectection of phenoloxidase in larvae, pupae and adults. Plasma protein samples (0.5 µl) from T. castaneum larva, lane 1; male pupa, lane 2; female pupa, lane 3; male adult, lane 4; and female adult, lane 5; M. sexta larval plasma, lane 6 (as a positive control) were separated by SDS-PAGE, transferred to nitrocellulose, and probed with anti-M. sexta phenoloxidase antibody. a Coomassie blue staining of the separated proteins. b Immunoblotting. Lane M represents molecular weight markers. Arrows indicate bands of the expected size for prophenoloxidase detected by prophenoloxidase antiserum

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BMC Research Notes

ISSN: 1756-0500

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