Table 1 Specifications of designed primers
Gene | Primer sequence(5′ to 3′) | Product length (bp) |
|---|---|---|
fnbAF | TGGTACTGATGAAGTTGATTTTAGAAC | 101 |
fnbAR | CATTATCCCAAGTTAAGGTATATCCTC | |
clfBF | CCGGTAGTAAATGCTGCTGTA | 103 |
clfBR | CACTTTGATTAGGGTCAAATGTAGTC | |
16sF | GGTCTGTAACTGACGCTGATGTG | 68 |
16sR | GTGGACTACCAGGGTATCTAATCCT | |
fnbBF | GGAGCGGCCTCAGTATTCTT | 201 |
fnbBR | AGTTGATGTCGCGCTGTATG | |
clfAF | CGCCGGTAACTGGTGAAGCT | 314 |
clfAR | TGCTCTCATTCTAGGCGCACTT |