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Fig. 3 | BMC Research Notes

Fig. 3

From: Periodic mild heat stimuli diminish extracellular matrix synthesis in pellet cultured human chondrocytes

Fig. 3

Collagen and proteoglycan accumulation. a Macro images and wet weight. Synthesized pellets on day 21 were subjected to macro photography (i) and wet weight measurement (ii). b Collagen accumulation. Collagen synthesis in pellets on day 21 was evaluated by picrosirius red staining (i) and hydroxyproline assay (ii). The values of collagen were normalized to DNA content. c Proteoglycan accumulation. Proteoglycan synthesis in pellets on day 21 was evaluated by safranin-O staining (i) and DMMB colorimetric assay (ii). The values of proteoglycan were normalized to DNA content. d DNA content. DNA content of pellets on day 21 was evaluated using the Quanti-iT™ PicoGreen assay. Values represent the means and standard deviations. * and ** indicates P < 0.05 and P < 0.01, respectively

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BMC Research Notes

ISSN: 1756-0500

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