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Table 1 Impact of extraction kits on total RNA yield, purity, integrity and small/miRNA yield

From: Simultaneous extraction of mRNA and microRNA from whole blood stabilized in tempus tubes

Characteristics Tempus Norgen MagMax p value
Total RNA yield (µg) 10.14 ± 0.73 12.04 ± 0.85 8.34 ± 0.76 0.008
Total RNA purity (260/280 ratio) 2.05 ± 0.01 2.09 ± 0.01 2.09 ± 0.01 0.027
Total RNA purity (260/230 ratio) 1.25 ± 0.05 1.98 ± 0.32 1.13 ± 0.06 < 0.0001
Total RNA integrity (RIN values) 8.70 ± 0.05 8.63 ± 0.05 6.68 ± 0.14 < 0.0001
Total RNA integrity (28S:18S ratio) 1.69 ± 0.04 1.54 ± 0.03 1.26 ± 0.06 < 0.0001
Small RNA yield (ng) 271.9 ± 34.2 344.8 ± 25.7 362.6 ± 24.8 Not significant
miRNA yield (ng) 24.7 ± 0.05 17.9 ± 1.6 39.3 ± 5.6 0.011
miRNA/Small RNA (%) 8.16 ± 1.6 5.17 ± 0.2 11.18 ± 1.8 0.014
  1. All values given are mean ± standard error of mean