Table 1 Impact of extraction kits on total RNA yield, purity, integrity and small/miRNA yield
From: Simultaneous extraction of mRNA and microRNA from whole blood stabilized in tempus tubes
Characteristics | Tempus | Norgen | MagMax | p value |
|---|---|---|---|---|
Total RNA yield (µg) | 10.14 ± 0.73 | 12.04 ± 0.85 | 8.34 ± 0.76 | 0.008 |
Total RNA purity (260/280 ratio) | 2.05 ± 0.01 | 2.09 ± 0.01 | 2.09 ± 0.01 | 0.027 |
Total RNA purity (260/230 ratio) | 1.25 ± 0.05 | 1.98 ± 0.32 | 1.13 ± 0.06 | < 0.0001 |
Total RNA integrity (RIN values) | 8.70 ± 0.05 | 8.63 ± 0.05 | 6.68 ± 0.14 | < 0.0001 |
Total RNA integrity (28S:18S ratio) | 1.69 ± 0.04 | 1.54 ± 0.03 | 1.26 ± 0.06 | < 0.0001 |
Small RNA yield (ng) | 271.9 ± 34.2 | 344.8 ± 25.7 | 362.6 ± 24.8 | Not significant |
miRNA yield (ng) | 24.7 ± 0.05 | 17.9 ± 1.6 | 39.3 ± 5.6 | 0.011 |
miRNA/Small RNA (%) | 8.16 ± 1.6 | 5.17 ± 0.2 | 11.18 ± 1.8 | 0.014 |