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Fig. 2 | BMC Research Notes

Fig. 2

From: Construction of a kiwifruit yeast two-hybrid cDNA library to identify host targets of the Pseudomonas syringae pv. actinidiae effector AvrPto5

Fig. 2

Y2H analysis of Psa AvrPto5 (bait) and AcHIPP26 (prey). Plate I—SDA/-Leu-Trp medium; Plate II—SDA/-Leu-Trp-His + 3-AT 1 mM medium; 1—Positive interaction control (Murine p53 (bait) + SV40 large T-antigen (prey); Clontech, USA); 2—Negative interaction control (Lamin (bait) + SV40 large T-antigen (prey); Clontech, USA); 3—Negative self-activation control (Empty bait and prey vectors); 4—Bait self-activation control (Psa AvrPto5 (bait) + Empty prey vector); 5—Prey self-activation control (Empty bait vector + AcHIPP26 (prey)); 6—True positive interaction control [33] (Pgy AvrB (Gene bank accession M21965) (bait) + AtRIN4 (AT3G25070) (prey)); 7—Psa AvrPto5 (bait) + AcHIPP26 (prey)

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