Fig. 2From: Thermostability and excision activity of polymorphic forms of hOGG1Thermolability of excision activity for hOGG1 variants. The hOGG1 variants’ enzyme activities were compared with or without thermal challenge. The top panel shows a representative gel image, in this case for the R46Q hOGG1 variant in the presence of non-specific DNA. In the lower panels, product formation, as measured from the glycosylase activity assay, is plotted as a function of time for each hOGG1 variant. Prior to the excision assay, hOGG1 variants were pre-incubated at either 37 °C (circles) or 4 °C (squares). The pre-incubation was carried out in the absence (open markers) or the presence (filled markers) of stoichiometric undamaged DNA. For each hOGG1 variant the glycosylase assay was replicated a minimum of three times at each condition. Error bars represent the standard deviationBack to article page