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Fig. 1 | BMC Research Notes

Fig. 1

From: Transformation improvement with the Standardized Pressure Agrobacterium Infiltration Device (SPAID)

Fig. 1

Attributes of the Standardized Pressure Agroinfiltration Device (SPAID). a The following labeled parts: (i) Plastic wrap, (ii) Cable ties holding syringe plunger, (iii) Syringe plunger soaking in 95% ethanol, (iv) Electronic analytical balance (in grams), (v) Metal plate, (vi) Dissecting microscope with coarse adjustment knob. b The syringe plunger mounted to the microscope nosepiece perpendicular to the microscope stage. c Agrobacterium solutions are transferred to a microcentrifuge tube. d Agrobacterium solutions are drawn into a syringe body. e Leaf explants are treated with Agrobacterium solution by rotating the coarse adjustment knob while monitoring reading on electronic scale

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