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Table 1 Agrobacterium concentration effects on transformation of strawberry leaf explants

From: Transformation improvement with the Standardized Pressure Agrobacterium Infiltration Device (SPAID)

OD600

% of explants with GFP callus

% of explants with GFP foci

0.1

18.9 ± 4.8 a

49 ± 9.5 a

0.5

21.1 ± 5.4 a

43.3 ± 6.0 a

0.8

21.1 ± 4.5 a

46.7 ± 8.2 a

1.2

18.9 ± 4.6 a

42.2 ± 5.8 a

  1. Thirty leaf explants for each concentration were used, and the experiment was repeated three times. The percent of surface area was calculated by using Quant Software. Data analyses were conducted with ANOVA by proc glm procedure SAS 9.4 with α = 0.05. The total surface area calculated is 0.401 cm2