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Table 2 Relationship between application pressure and stable GFP-positive foci on leaf explants from four plant species

From: Transformation improvement with the Standardized Pressure Agrobacterium Infiltration Device (SPAID)

Scale reading (g) kPa Percent of explants with stable GFP foci
Strawberry Tobacco Arabidopsis
100 54 8.89 ± 2.10 a 84.50 ± 8.96 a 35.55 ± 4.33 a
200 109 9.68 ± 2.10 a 80.49 ± 6.95 a 22.06 ± 3.59 a
300 163 17.64 ± 4.10 ab 77.66 ± 6.18 a 24.49 ± 7.62 a
400 218 17.97 ± 11.1 ab 77.97 ± 7.34 a 21.92 ± 1.42 a
500 273 49.04 ± 11.1 c 85.04 ± 12.91 ab 16.09 ± 5.05 ab
600 327 40.49 ± 5.65 c 70.49 ± 3.68 a 9.86 ± 9.80 ab
700 382 31.33 ± 5.14 b 40.33 ± 5.92 b 8.23 ± 3.83 b
800 437 37.67 ± 10.36 bc 45.67 ± 9.90 b 11.35 ± 1.63 b
900 491 7.49 ± 1.95 a 27.49 ± 2.41 c 8.46 ± 3.47 b
1000 546 11.00 ± 4.35 a 21.00 ± 6.38 c 9.66 ± 2.23 b
  1. GFP positive callus events were counted 2 weeks after cocultivation with different syringe pressures applied on strawberry, tobacco or Arabidopsis leaf explants. Three separate experimental replicates were performed containing 30–40 explants each. Data analyses were conducted with ANOVA by proc glm procedure SAS 9.4 with α = 0.05