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Table 2 Relationship between application pressure and stable GFP-positive foci on leaf explants from four plant species

From: Transformation improvement with the Standardized Pressure Agrobacterium Infiltration Device (SPAID)

Scale reading (g)

kPa

Percent of explants with stable GFP foci

Strawberry

Tobacco

Arabidopsis

100

54

8.89 ± 2.10 a

84.50 ± 8.96 a

35.55 ± 4.33 a

200

109

9.68 ± 2.10 a

80.49 ± 6.95 a

22.06 ± 3.59 a

300

163

17.64 ± 4.10 ab

77.66 ± 6.18 a

24.49 ± 7.62 a

400

218

17.97 ± 11.1 ab

77.97 ± 7.34 a

21.92 ± 1.42 a

500

273

49.04 ± 11.1 c

85.04 ± 12.91 ab

16.09 ± 5.05 ab

600

327

40.49 ± 5.65 c

70.49 ± 3.68 a

9.86 ± 9.80 ab

700

382

31.33 ± 5.14 b

40.33 ± 5.92 b

8.23 ± 3.83 b

800

437

37.67 ± 10.36 bc

45.67 ± 9.90 b

11.35 ± 1.63 b

900

491

7.49 ± 1.95 a

27.49 ± 2.41 c

8.46 ± 3.47 b

1000

546

11.00 ± 4.35 a

21.00 ± 6.38 c

9.66 ± 2.23 b

  1. GFP positive callus events were counted 2 weeks after cocultivation with different syringe pressures applied on strawberry, tobacco or Arabidopsis leaf explants. Three separate experimental replicates were performed containing 30–40 explants each. Data analyses were conducted with ANOVA by proc glm procedure SAS 9.4 with α = 0.05