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Table 1 Analyses of candidate reference genes by BestKeeper, GeNorm and NormFinder algorithms

From: Reference genes for gene expression studies targeting sugarcane infected with Sugarcane mosaic virus (SCMV)

BestKeepera        
Gene rankingb SAND UK GAPDH UBC18 RPL1 UBQ1 ACT
N 24 24 24 24 24 24 24
SD [± CP] 0.21 0.25 0.28 0.38 0.59 1.02 1.04
CV [% CP] 0.72 0.97 1.18 1.28 1.98 2.99 3.24
BestKeeperc        
Gene rankingb SAND UK GAPDH UBC18 RPL1 UBQ1 ACT
UK 0.456
  0.025
GAPDH − 0.563 − 0.611
  0.004 0.002
UBC18 0.473 0.690 − 0.367
  0.019 0.001 0.078
RPL1 0.259 0.163 − 0.393 0.320
  0.221 0.444 0.058 0.128
UBQ1 0.151 − 0.126 0.036 − 0.232 − 0.345
  0.479 0.555 0.867 0.275 0.099
ACT − 0.330 − 0.419 0.781 − 0.353 − 0.773 0.419
  0.115 0.041 0.001 0.091 0.001 0.041
Coeff. of corr. [r]d 0.211 − 0.004 0.365 0.130 − 0.299 0.760 0.636
P-value 0.323 0.984 0.079 0.542 0.156 0.001 0.001
BestKeepere SAND UK   UBC18    
Coeff. of corr. [r] 0.705 0.867   0.910    
P-value 0.001 0.001   0.001    
GeNorm        
Gene rankingb UBC18 UK SAND GAPDH RPL1 UBQ1 ACT
Stablity (M) 0.224 0.224 0.276 0.578 0.691 1.296 1.343
NormFinder        
Gene rankingb SAND UK GAPDH UBC18 RPL1 UBQ1 ACT
Stability 0.227 0.274 0.279 0.381 0.539 0.677 0.709
Best gene combination of two genes SAND   GAPDH     
Stability 0.181   0.181     
  1. aBestKeeper SD statistics of all seven candidate reference genes
  2. bRanking from the most to the least stable gene (left to right)
  3. cRepeated pair-wise correlation analysis among candidate reference genes
  4. dCoefficient of correlation with the BestKeeper index
  5. eCoefficient of correlation with the BestKeeper index after the exclusion of candidate reference genes with standard dev (SD) above 1.0 and with low Pearson correlation (r) values