Fig. 2
From: Mutagenesis of seed storage protein genes in Soybean using CRISPR/Cas9
CRISPR/Cas9-mediated disruption of soybean storage protein genes in hairy roots. DNA sequencing peaks showing successful gene editing in target regions of Glyma20g28650/Glyma20g28660 (a), Glyma030g32030 (b), and Glyma19g34780 (c). Sequencing result from WT served as the negative control. Red triangles point to the putative cutting sites by Cas9. Cloning and Sequencing results of mutant alleles of Glyma20g28650/Glyma20g28660 (d), Glyma030g32030 (e), and Glyma19g34780 (f). The top row is the schematic representation of genomic locus. Black boxes and black lines represent exons and UTRs, respectively. Red vertical line indicates the position of sgRNAs. Letters D and S indicate the number of nucleotides deleted and substituted, respectively. The asterisks indicate the numbers of independent clones sequenced