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Table 1 Summary of mutations generated for each sgRNA

From: Mutagenesis of seed storage protein genes in Soybean using CRISPR/Cas9

Genes ID for sgRNA gRNA-PAMa No. hairy roots genotyped No. hairy roots w. mutations detected % mutation
Glyma.20g148400 g1 5′-CCTTCTGATGAGGTGGGCGTGGG-3′ 17 1 5.8
Glyma.20g146200 g1 5′-GGACAATCCGGTAGTCTCGAAGG-3′ 46 0  
g2 5′-ACAGAAGCAGAAACAGGAAGAGG-3′ 37 0  
g3 5′-GGATTCTCTGGGCATCGCCACCC-3′ 42 0  
Glyma.10g246300 g1 5′-TCTCGCTATTGCAACTTCGGAGG-3′ 27 0  
g2 5′-CAGTGTTGTGGATATGAACGAGG-3′ 32 0  
g3 5′-AGAAGAAGAAGACCAAGACGAGG-3′ 21 0  
Glyma.20g148200 g1 5′-GGATTCTCTGGGCATCGCCAGGG-3′ 75 0  
g2 5′-ACTCTCTTTGAGAACCAAAACGG-3′ 63 0  
g3 5′-GGACAATCCGGTAGTCTCGAAGG-3′ 28 0  
Glyma.10g037100 g1 5′-CATCTTACTCACCTTATCCCCGG-3′ 99 0  
g2 5′-GACGTACTCGTGATTCCTCCGG-3′ 54 0  
g3 5′-CTCCATTCGCGGCTCTTGCGAGG-3′ 42 0  
Glyma.03g163500 g1 5′-GATAACCGTATAGAGTCAGAAGG-3′ 26 1 3.8
Glyma.19g164900 g1 5′-GATAACCGTATAGAGTCAGAAGG-3′ 32 14 43.7
Glyma.13g123500 g1 5′-GACCACCGCGTTGAGTCCGAAGG-3′ 38 0  
g2 5′-GACGTACTAGTGATTCCTCCTGG-3′ 64 0  
g3 5′-TCGGGCCTGCTTGGTCGCTGTGG-3′ 48 0  
Glyma.19g164800 g1 5′-GACAACCTCATCGAATCCCAAGG-3′ 49 0  
g2 5′-AGCGTGGCCTACGTGACGAGTGG-3′ 76 0  
g3 5′-TCGGTGTTCAGCGGCGCTGTTGG-3′ 49 0  
  1. aThe PAM sequences are highlighted in bold