From: Mutagenesis of seed storage protein genes in Soybean using CRISPR/Cas9
Genes | ID for sgRNA | gRNA-PAMa | No. hairy roots genotyped | No. hairy roots w. mutations detected | % mutation |
---|---|---|---|---|---|
Glyma.20g148400 | g1 | 5′-CCTTCTGATGAGGTGGGCGTGGG-3′ | 17 | 1 | 5.8 |
Glyma.20g146200 | g1 | 5′-GGACAATCCGGTAGTCTCGAAGG-3′ | 46 | 0 |  |
g2 | 5′-ACAGAAGCAGAAACAGGAAGAGG-3′ | 37 | 0 |  | |
g3 | 5′-GGATTCTCTGGGCATCGCCACCC-3′ | 42 | 0 |  | |
Glyma.10g246300 | g1 | 5′-TCTCGCTATTGCAACTTCGGAGG-3′ | 27 | 0 |  |
g2 | 5′-CAGTGTTGTGGATATGAACGAGG-3′ | 32 | 0 |  | |
g3 | 5′-AGAAGAAGAAGACCAAGACGAGG-3′ | 21 | 0 |  | |
Glyma.20g148200 | g1 | 5′-GGATTCTCTGGGCATCGCCAGGG-3′ | 75 | 0 |  |
g2 | 5′-ACTCTCTTTGAGAACCAAAACGG-3′ | 63 | 0 |  | |
g3 | 5′-GGACAATCCGGTAGTCTCGAAGG-3′ | 28 | 0 |  | |
Glyma.10g037100 | g1 | 5′-CATCTTACTCACCTTATCCCCGG-3′ | 99 | 0 |  |
g2 | 5′-GACGTACTCGTGATTCCTCCGG-3′ | 54 | 0 |  | |
g3 | 5′-CTCCATTCGCGGCTCTTGCGAGG-3′ | 42 | 0 |  | |
Glyma.03g163500 | g1 | 5′-GATAACCGTATAGAGTCAGAAGG-3′ | 26 | 1 | 3.8 |
Glyma.19g164900 | g1 | 5′-GATAACCGTATAGAGTCAGAAGG-3′ | 32 | 14 | 43.7 |
Glyma.13g123500 | g1 | 5′-GACCACCGCGTTGAGTCCGAAGG-3′ | 38 | 0 |  |
g2 | 5′-GACGTACTAGTGATTCCTCCTGG-3′ | 64 | 0 |  | |
g3 | 5′-TCGGGCCTGCTTGGTCGCTGTGG-3′ | 48 | 0 |  | |
Glyma.19g164800 | g1 | 5′-GACAACCTCATCGAATCCCAAGG-3′ | 49 | 0 |  |
g2 | 5′-AGCGTGGCCTACGTGACGAGTGG-3′ | 76 | 0 |  | |
g3 | 5′-TCGGTGTTCAGCGGCGCTGTTGG-3′ | 49 | 0 |  |