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Table 1 Summary of mutations generated for each sgRNA

From: Mutagenesis of seed storage protein genes in Soybean using CRISPR/Cas9

Genes

ID for sgRNA

gRNA-PAMa

No. hairy roots genotyped

No. hairy roots w. mutations detected

% mutation

Glyma.20g148400

g1

5′-CCTTCTGATGAGGTGGGCGTGGG-3′

17

1

5.8

Glyma.20g146200

g1

5′-GGACAATCCGGTAGTCTCGAAGG-3′

46

0

 

g2

5′-ACAGAAGCAGAAACAGGAAGAGG-3′

37

0

 

g3

5′-GGATTCTCTGGGCATCGCCACCC-3′

42

0

 

Glyma.10g246300

g1

5′-TCTCGCTATTGCAACTTCGGAGG-3′

27

0

 

g2

5′-CAGTGTTGTGGATATGAACGAGG-3′

32

0

 

g3

5′-AGAAGAAGAAGACCAAGACGAGG-3′

21

0

 

Glyma.20g148200

g1

5′-GGATTCTCTGGGCATCGCCAGGG-3′

75

0

 

g2

5′-ACTCTCTTTGAGAACCAAAACGG-3′

63

0

 

g3

5′-GGACAATCCGGTAGTCTCGAAGG-3′

28

0

 

Glyma.10g037100

g1

5′-CATCTTACTCACCTTATCCCCGG-3′

99

0

 

g2

5′-GACGTACTCGTGATTCCTCCGG-3′

54

0

 

g3

5′-CTCCATTCGCGGCTCTTGCGAGG-3′

42

0

 

Glyma.03g163500

g1

5′-GATAACCGTATAGAGTCAGAAGG-3′

26

1

3.8

Glyma.19g164900

g1

5′-GATAACCGTATAGAGTCAGAAGG-3′

32

14

43.7

Glyma.13g123500

g1

5′-GACCACCGCGTTGAGTCCGAAGG-3′

38

0

 

g2

5′-GACGTACTAGTGATTCCTCCTGG-3′

64

0

 

g3

5′-TCGGGCCTGCTTGGTCGCTGTGG-3′

48

0

 

Glyma.19g164800

g1

5′-GACAACCTCATCGAATCCCAAGG-3′

49

0

 

g2

5′-AGCGTGGCCTACGTGACGAGTGG-3′

76

0

 

g3

5′-TCGGTGTTCAGCGGCGCTGTTGG-3′

49

0

 
  1. aThe PAM sequences are highlighted in bold