Fig. 2

Immunohistochemical analysis in mouse lungs. a Representative image of immunohistochemical analysis of HGF. Arrows indicate HGF-positive alveolar macrophages (AMs). Arrow heads indicate HGF-negative AMs. Right side graph indicates positive cells per field. Data are presented as mean ± SE (n = 3). There were significant differences between PBS-treated and LPS-treated control mice, and between LPS-treated control and β-cell-depleted hyperglycemic mice (P < 0.05). b Typical image of immunohistochemical analysis using anti-PCNA polyclonal antibody. Upper picture, control mouse; lower picture, β-cell-depleted hyperglycemic mouse. Right side graph indicates that the numbers of positive cells per field were significantly different between LPS-treated control and β-cell-depleted hyperglycemic mice (n = 3, P < 0.05)