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Fig. 1 | BMC Research Notes

Fig. 1

From: Novel binding partners for Prenylated Rab Acceptor 1 identified by a split-ubiquitin yeast two-hybrid screen

Fig. 1

Split ubiquitin yeast two-hybrid mechanism and screening strategy. a LexA-VP16-CUB-PRA1 was used as bait in yeast to screen against NUB-X mouse neuronal cDNA library. An interaction leads to reconstitution of Ubiquitin and cleavage of LexA-VP16 by ubiquitin specific proteases (UBPs), which migrates to the nucleus to transcribe target genes. b Summary of yeast-two hybrid screening strategy. c PRA1 interaction with (clockwise from the 9 o’clock position): Mcm3ap, Ppargc1a, Appl1, Top1, Chmp2b, Calm3, Atpase6, and Rangrf leads to growth on dropout media (SD–LEU–HIS–TRP 10 mM 3-aminotriazole). CUB amino acids 34–76 of ubiquitin, NUB amino acids 1–38 of ubiquitin

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