Fig. 2

BiFC assay in COS-7 cells confirms three interactions revealed by the split-ubiquitin yeast two-hybrid screen. PRA1-VN173 was co-transfected with a Lamin-A-VC155 (left) and an mCherry transfection control construct (right), b LAMTOR2-VC155, c ζ1-COP-VC155, and d Calmodulin-3-VC155. All three identified potential interacting proteins lead to fluorescence while the Lamin-A negative control did not. Blue: DAPI, Red: mCherry, Yellow: Venus, Scale bar: 10 μm