Fig. 3
From: Mebendazole-induced M1 polarisation of THP-1 macrophages may involve DYRK1B inhibition
Flow cytometry analysis of CD80 and CD163 expression in response to 10 µM AZ191 (a) after 48 h of incubation. In b the effect of AZ191 and MBZ on ERK phosphoprotein activation in PMA differentiated THP-1 cells is shown. ERK activity was measured after 1 h exposure of the drugs. The results are expressed as relative fluorescence units (RFU) and presented as mean values ± standard deviation for duplicate measurements. See “Methods” for details. Measurements were performed by a Luminex Magpix instrument and a ERK phosphoprotein kit from Biorad. A lysate of EGF-treated HEK293 cells provided in the kit served as positive control