Table 2 Primer sequences, motifs, and characteristics of the 14 microsatellite loci developed and optimized from the turkey vulture (Cathartes aura)
From: Development of microsatellite loci for two New World vultures (Cathartidae)
Locus | Panel | Dye label | Primer sequence (5′–3′); F, forward; R, reverse | Repeat motif | n | Allele size range (bp) | N A | H O | H E |
|---|---|---|---|---|---|---|---|---|---|
TUVU-21b | A | 6-FAM | F: TTGTTTGGCTCCATGTTTGG | CT(10) AC(8) AC(10) | 30 | 202–210 | 3 | 0.20 | 0.39 |
R: ACACCCATTCAAATGCAAGC | |||||||||
TUVU-06 | A | 6-FAM | F: GAGTCAGCAATGGTGGTTGC | GAA(25) GAA(8) | 30 | 326–533 | 17 | 0.77 | 0.83 |
R: ACTGTAGCAGTGACGGCAGC | |||||||||
TUVU-31 | A | VIC | F: AAGTAAATAGCTGTCTAACTGTTCATCC | TG(15) AT(8) | 30 | 122–152 | 8 | 0.73 | 0.81 |
R: CTTTCATGCCTTGATTTCCC | |||||||||
TUVU-23 | A | NED | F: GAAACGGTATTTGCCTTGCC | TTCC(12) | 30 | 155–161 | 6 | 0.60 | 0.60 |
R: AAAACTCCAAGGGGAGGAGG | |||||||||
TUVU-39b | A | PET | F: GGTTCAGGTGAGAGAAACCCC | AAAG(23) | 30 | 188–311 | 20 | 0.77 | 0.90 |
R: GAAAACCCCTCTGGGAAACC | |||||||||
TUVU-14a,b | B | 6-FAM | F: CCTAGTCCGGAAACACAGGG | ATT(14) ATT(6) | 30 | 347–371 | 7 | 0.50 | 0.75 |
R: TTAAACTGAAATGTGTGAAGAAGCG | |||||||||
TUVU-07 | B | 6-FAM | F: TGGGATGTGAAGGAGAACAGC | GGAA(28) | 30 | 160–226 | 15 | 0.87 | 0.92 |
R: TGACTCCTGTACAAAATTAGATCCTTCC | |||||||||
TUVU-45 | B | VIC | F: AATAATCCATGAGCACCAGGC | GTTT(14) | 30 | 131–158 | 7 | 0.50 | 0.45 |
R: CCCATAAACTCAAGCATTGGC | |||||||||
TUVU-03 | B | PET | F: AGGTTCATTAGCAGAGGCGG | AAAG(9) AAAG(18) | 30 | 261–375 | 30 | 1.00 | 0.96 |
R: GTGGCAGAAAGAAGCTGAAGG | |||||||||
TUVU-01 | C | 6-FAM | F: TCATACACTGGTCGTTCGCC | TC(6) CTTT(24) CTTT(12) | 30 | 290–640 | 47 | 1.00 | 0.99 |
R: ATTGAAATGCCCTACAGACGG | |||||||||
TUVU-18 | C | VIC | F: GGTTCTGCTGATTTCAACTTTGC | TAA(11) TGA(9) | 30 | 240–249 | 4 | 0.50 | 0.47 |
R: TTCACCACAGGAAACCAAAGC | |||||||||
TUVU-37 | C | NED | F: GCTGGTTTTGAACAGTGAGGG | AAAG(27) AAAG(8) | 30 | 263–415 | 34 | 0.93 | 0.97 |
R: TTACAGGTGGGGAATCTCAGG | |||||||||
TUVU-33 | C | PET | F: GCAAATCAGCCTCTGGTGG | TA(13) TA(6) | 30 | 330–339 | 9 | 0.77 | 0.83 |
R: TTAACTTGGAGGCCAGGAGG | |||||||||
TUVU-36a,b | C | PET | F: CACACGCACACAATGCACC | GC(8) | 30 | 169–173 | 3 | 0.07 | 0.45 |
R: CACTGCGCGAGTGTGAGG |