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Table 3 Multiplex PCR characteristics for the three optimized black vulture (Coragyps atratus) microsatellite panels (BLVU) and the three optimized from the turkey vulture (Cathartes aura) microsatellite panels (TUVU)

From: Development of microsatellite loci for two New World vultures (Cathartidae)

Panel

Reaction volume (µL)

Ta (°C)

No. of cycles

Panel

Reaction volume (µL)

Ta (°C)

No. of cycles

BLVU-A

10.0

58

32

TUVU-A

13.0

58

34

BLVU-B

9.0

58

32

TUVU-B

16.0

58

37

BLVU-C

9.0

58

32

TUVU-C

15.0

58

44

  1. Ta is the annealing temperature and no. of cycles is the number of amplification cycles in the thermal cycler protocol