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Table 3 Multiplex PCR characteristics for the three optimized black vulture (Coragyps atratus) microsatellite panels (BLVU) and the three optimized from the turkey vulture (Cathartes aura) microsatellite panels (TUVU)

From: Development of microsatellite loci for two New World vultures (Cathartidae)

Panel Reaction volume (µL) Ta (°C) No. of cycles Panel Reaction volume (µL) Ta (°C) No. of cycles
BLVU-A 10.0 58 32 TUVU-A 13.0 58 34
BLVU-B 9.0 58 32 TUVU-B 16.0 58 37
BLVU-C 9.0 58 32 TUVU-C 15.0 58 44
  1. Ta is the annealing temperature and no. of cycles is the number of amplification cycles in the thermal cycler protocol