Fig. 3

Purity and homogeneity analysis of HA samples by HPSEC-CAD. a Shows the overlay of HPSEC chromatograms of HA GPC standards of varying molecular weights (pink: 8–15 kDa; blue: 10–30 kDa; black: 30–50 kDa). b, c Represent HPSEC chromatograms of commercially available HA products. Although b is predominantly composed of a single peak, the shape of the peak indicates that it might not be a homogeneous fraction. c Contains two non-symmetrical peaks indicating that it is a heterogeneous sample