Table 1 Overview of RT-qPCR approaches tested

1

5 µL plasma + 5 µL denaturing buffer

Incubation at 75 °C for 5 min, cool on ice

Ad 2 µL spike^a

Centrifugation at 10,000g for 10 min at 4 °C

6 µL plasma preparation in a total volume of 15 µL

None

Zhao et al. [9]

2

2.5 µL plasma + 2.5 µL denaturing buffer

Ad 1 µL spike^a

5 µL plasma preparation in a total volume of 15 µL

Centrifugation at 10,000g for 10 min

Asaga et al. [7]

Zheng et al. [12]

3

2.5 µL plasma + 2.5 µL denaturing buffer

Ad RNase inhibitor and RT-primer pool

Incubation at 70 °C for 10 min

Ad rest of RT-reaction mixture and 1 µL spike^a (total 15 µL)

Centrifugtion at 10,000g for 10 min

Liu et al. [8]

4

5 µL plasma + 5 µL denaturing buffer

Ad RNase inhibitor and 2 µL spike^a

Incubation at 70 °C for 10 min, cool on ice

Centrifugation at 10,000g for 10 min at 4 °C

6 µL plasma preparation in a total volume of 15 µL

None