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Table 2 Ct-values obtained by approach number 3 using the buffer with pH 8

From: Quantification of microRNA in plasma using probe based TaqMan assays: is microRNA purification required?

  miR-126 miR-16 miR-92a
Sample 1 Sample 2 Sample 1 Sample 2 Sample 1 Sample 2
PPP 38.4 35.9 37.1 35.0 30.5 29.5
PPP 1:10 39.7 39.1   34.0 34.5 32.4
PPP 1:100 38.5 38.3 32.5 29.8 34.7 32.7
Serum 37.9 40.9 37.0 40.6 31.5 37.6
Serum 1:10 40.1    37.8 33.4 36.9
Serum 1:100 38.0 38.4 32.6 35.0 32.5 34.8
  1. The table provides Ct-values obtained in undiluted and diluted PPP and serum samples from two individuals. Empty cells represent undetermined values