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Table 2 Ct-values obtained by approach number 3 using the buffer with pH 8

From: Quantification of microRNA in plasma using probe based TaqMan assays: is microRNA purification required?

 

miR-126

miR-16

miR-92a

Sample 1

Sample 2

Sample 1

Sample 2

Sample 1

Sample 2

PPP

38.4

35.9

37.1

35.0

30.5

29.5

PPP 1:10

39.7

39.1

 

34.0

34.5

32.4

PPP 1:100

38.5

38.3

32.5

29.8

34.7

32.7

Serum

37.9

40.9

37.0

40.6

31.5

37.6

Serum 1:10

40.1

  

37.8

33.4

36.9

Serum 1:100

38.0

38.4

32.6

35.0

32.5

34.8

  1. The table provides Ct-values obtained in undiluted and diluted PPP and serum samples from two individuals. Empty cells represent undetermined values