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Fig. 1 | BMC Research Notes

Fig. 1

From: Utility of I-SceI and CCR5-ZFN nucleases in excising selectable marker genes from transgenic plants

Fig. 1

Expression of I-SceI and ZFN in rice. a, b Overexpression and inducible constructs of I-SceI or ZFN contain ZmUbi1 for constitutive overexpression or GmHSP17.5E for HS-inducible expression with nos 3′ as transcription termination sequence. c, d Real-time quantitative PCR analysis on total RNA isolated from the rice lines expressing HS inducible I-SceI or ZFN gene. Relative expression against wild-type control is shown for each line. Bars show mean of two treatments with standard errors. Red and blue bars represent HS and room temperature (RT) samples, respectively. Note that ZFN expression at RT was close to the wild-type controls

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