Fig. 1

Schematic diagram of construction of bilayer model to mimic RPE/BM/choroid. The primary retinal pigment epithelial and choroidal endothelial cells were isolated from donor eyes. The primary cells were grown on either side of the transwell insert. To create the pathological milieu, these bilayer cells were treated with VEGF and their barrier permeability was checked using FITC-dextran conjugate