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Fig. 2 | BMC Research Notes

Fig. 2

From: Optimization of an in vitro bilayer model for studying the functional interplay between human primary retinal pigment epithelial and choroidal endothelial cells isolated from donor eyes

Fig. 2

Images of human primary ocular cells. a Top left panel shows human microvascular choroidal endothelial cells (hCEC) freshly isolated (P0) and the first passage (P1) on the right side. Bottom left and right panels show the human retinal pigment epithelial (hRPE) cells at P0 and P1 respectively. b Semi-quantitative RT-PCR was done to show the presence of von willebrand factor transcripts in hCEC and HUVEC. c RT-PCR for Cytokeratin-18 in the primary hRPE and ARPE19 cells was done. GAPDH was used as an internal control (n = 3). d Immunofluorescence staining of von willebrand factor in hCEC and Cytokeratin-18 in hRPE (n = 3). e Tube formation assay was done on matrigel using hCEC. f PEDF levels in the medium from upper and lower chamber of insert grown hRPE was measured by ELISA, p = 0.006 (n = 3). g VEGF ELISA was performed in the medium taken from upper and lower chamber of hRPE p = 0.02 (n = 3)

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