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Table 1 Alternative splicing of NCoR-1 and NCoR-2 corepressors

From: Evolution of NCoR-1 and NCoR-2 corepressor alternative mRNA splicing in placental mammals

Species NCoR-1 NCoR-2
28b+ 28b− 37b+ 37b− 45a+ 45a− 45− 28b+ 28b− 40b+ 40b− 47b+ 47b− 47−
Alligator 100 ND 100 ND 100 ND ND 100 ND 29.0 (4.4) 71.0 (4.4) 22.4 (5.3) 40.0 (6.0) 37.6 (5.3)
Bat 100 ND 73.1 (8.7) 26.9 (8.7) 100 ND ND 100 ND 52.5 (9.0) 47.5 (9.0) * * *
Cat 100 ND 59.8 (1.7) 40.2 (1.7) 49.0 (2.3) ND 51.0 (2.3) 100 ND 25.1 (1.2) 74.9 (1.2) 100 ND ND
Chicken 100 ND 100 ND 100 ND ND 100 ND ND 100 24.6 (3.8) 41.8 (3.9) 33.6 (3.6)
Dog 100 ND 64.4 (3.9) 35.6 (3.9) 59.5 (2.2) ND 40.5 (2.2) 100 ND 21.7 (2.6) 78.3 (2.6) 28.0 (4.5) 72.0 (4.5) ND
Elephant 100 ND 89.4 (3.3) 10.6 (3.3) 100 ND ND 100 ND 30.4 (2.9) 69.6 (2.9) 24.3 (3.1) 46.0 (5.3) 29.7 (5.5)
Horse 100 ND 69.3 (6.8) 30.7 (6.8) 100 ND ND 100 ND 26.8 (5.4) 73.2 (5.4) ND 46.4 (6.6) 53.6 (6.6)
Human 100 ND 70.2 (5.5) 29.8 (5.5) 100 ND ND 86.0 (2.5) 14.0 (2.5) 17.5 (3.9) 82.5 (3.9) 20.4 (1.8) 53.6 (1.5) 26.0 (1.9)
Mouse 76.2 (0.2) 23.8 (0.2) 54.2 (2.4) 45.8 (2.4) 88.8 (0.9) 11.2 (0.9) ND 86.9 (2.7) 13.1 (2.7) 32.9 (2.3) 67.1 (2.3) 17.2 (1.8) 61.2 (4.2) 21.6 (2.8)
Pig 100 ND 55.0 (1.5) 45.0 (1.5) 100 ND ND 100 ND 27.9 (0.6) 72.1 (0.6) ND 64.5 (1.7) 35.5 (1.7)
Rabbit 100 ND 63.8 (1.3) 36.2 (1.3) 100 ND ND 100 ND 15.2 (1.1) 84.8 (1.1) ND 54.4 (3.4) 45.6 (3.4)
Rat 73.2 (1.7) 26.8 (1.7) 58.0 (3.6) 42.0 (3.6) 100 ND ND 100 ND 32.8 (2.0) 67.2 (2.0) 17.3 (2.1) 60.3 (1.6) 22.3 (3.1)
Rhesus 100 ND 63.8 (5.4) 36.2 (5.4) 100 ND ND 69.7 (2.6) 30.2 (2.6) 19.5 (3.0) 80.5 (3.0) 19.5 (2.1) 58.6 (6.1) 22.0 (6.4)
  1. Alternative mRNA splicing was determined as described in "Methods" and Additional file 1 from RNA isolated from peripheral blood from each species listed (Alligator, Alligator mississippiensis; Bat, Eidolon helvum; Cat, Felis silvestris catus; Chicken, Gallus gallus domesticus; Dog, Canis lupus familiaris; Elephant, Loxodonta africana; Horse, Equus ferus caballus, Human, Homo sapien; Mouse, Mus musculus; Pig, Sus scrofa domesticus; Rabbit, Oryctolagus cuniculus; Rat, Rattus norvegicus; Rhesus, Macaca mulatta). Results from an individual of each species are presented. The relative abundance of each alternative-splice variant from each splice site analyzed is presented as a percent of the total splice variants expressed from that site (sum = 100%)
  2. ND = not detected (limits of detection were estimated as < 2% of total expression at each splice site, the limits of accurate quantitation as > 5%). The structures of the alternative-spliced transcripts are detailed in [23] except for the NCoR-1 exon 45− splice variant, which was newly recognized from the current analysis and was confirmed by sequence analysis of the PCR products. The mean and (standard error) of 3 or more analyzes are shown for each value
  3. * Data not available. See “Limitations” and Additional file 1 for an expanded discussion of the sensitivity and accuracy of this RT-PCR methodology