Fig. 3

a Heatmap of regulated genes related to phagocytosis in the corpus callosum (CC), cerebellum (Cb) and cortex (Cx) at different ages [postnatal day (P)7, 10, 42]. b Heatmap of known priming genes in the CC, Cb and Cx at P7, P10 and P42. Asterisks highlighting genes confirmed on protein level; Representative immunofluorescence pictures of wildtype mice depicting SPP1 [red (c, e)], CLEC7a [white (c, e)] and CD206 [white (d)] expression in IBA-1⁺ microglia (green) in the CC (c, d), Cx (e left) and Cb (e right) at P7, P10 and P42. Scale bar 50 µm; blue = DAPI; arrow indicating IBA-1⁺/SPP1⁺ cell in Cb; N = 3–4 mice per timepoint