Fig. 1From: The pluripotency transcription factor Nanog represses glutathione reductase gene expression in mouse embryonic stem cellsGsr is upregulated in ESCs cultured without LIF. R1 ESCs were cultured under standard conditions in the presence of LIF, or in the absence of LIF for 4 days. a Representative pictures of cells cultured with LIF (+LIF) and without LIF (−LIF) after 4 days of treatment. Scale bars: 100 µm. b RNA was extracted and mRNA levels of Oct4, Nanog and Gsr were measured by RT-qPCR. Gene expression was normalized to the geometrical mean of Gapdh and Pgk1 expression and referred to the control condition (with LIF, shown as a line). Results are shown as mean ± SEM of four independent experiments (different letters indicate significant differences, p < 0.05). c Representative immunostaining of Nanog for R1 ESCs cultured in the presence of LIF (+LIF) or in the absence of LIF for 4 days (−LIF). Nuclei were counterstained with DAPI. Scale bars: 20 μm. The nuclear intensities were quantified and represented as dot plots, mean ± SEM are indicated, *p < 0.05Back to article page