Fig. 2
From: Dynamics of actin polymerisation during the mammalian single-cell wound healing response
Mammalian single-cell repair does not involve myosin enrichment at the wound borders. a Maximum intensity projections of selected micrographs of the CellMask and MRLC signals following laser-mediated ablation of the PM of HeLa cells. b Normalized MLRC fluorescent signal intensity (MRLC FIR) at the wound site relative to control regions. Mean and SEM shown of n = 12 cells. c Kymograph of the MRLC signal before and after laser-mediated ablation of the PM. Cell and targeted area is the same as in the one shown in a. d Resliced cortical volume (each 10 μm) of selected time-points of the wounding assay shown in a, c. Targeted area (95 μm2) is represented by the red circle found on the pre-wounded, PM micrograph. The line scan region used for the creation of the kymograph and resliced volumes correspond to the cyan line (22 μm) displayed on the 0 s, MRLC micrograph. The cell shown in a, c, and d is representative of n = 12 wounding assays, all of which were used to prepare b. Scale bars = 10 μm