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Table 2 Processed samples detected using the proposed protocol

From: Rapid identification of Aconitum plants based on loop-mediated isothermal amplification assay

  Non-processed sample (leaf) Boiled sample Fried sample
5 min 10 min 1 min 3 min 10 min
Ac. japonicum subsp. subcuneatum + (+) + (+) + (+) + (+) + (+) − (−)
Anemone flaccida − (−) − (−) − (−) − (−) − (−) − (−)
  1. Dried leaves were processed by boiling for 5 or 10 min or frying for 1, 3, and 10 min. The symbol ‘+’ indicates successful amplification within 20 min. No amplification within 20 min was denoted by the symbol ‘−’. The results of processed samples that were treated with artificial gastric juice for 2 h are shown in parentheses