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Table 2 Processed samples detected using the proposed protocol

From: Rapid identification of Aconitum plants based on loop-mediated isothermal amplification assay

 

Non-processed sample (leaf)

Boiled sample

Fried sample

5 min

10 min

1 min

3 min

10 min

Ac. japonicum subsp. subcuneatum

+ (+)

+ (+)

+ (+)

+ (+)

+ (+)

− (−)

Anemone flaccida

− (−)

− (−)

− (−)

− (−)

− (−)

− (−)

  1. Dried leaves were processed by boiling for 5 or 10 min or frying for 1, 3, and 10 min. The symbol ‘+’ indicates successful amplification within 20 min. No amplification within 20 min was denoted by the symbol ‘−’. The results of processed samples that were treated with artificial gastric juice for 2 h are shown in parentheses