Fig. 2

Effect of the peptides DL18 and LL18 (in comparison to commercially available peptide inhibitor 3396) on N1E and R3 cells under different apoptogenic stimuli. Rows 1–3 (left) and 6–12 (right) are fluorescent microscopy (×10 objective; superimposed scale bars are 100 µm) photographs after double staining with Calcein A and Ethidium homodimer. Rows 4–5—Western blotting: Upper line—loading control checked with antibodies against HSC-70, Lower line—caspase cleavage detected with primary antibodies against active caspase 3. Treatments: untreated control, Staurosporine (100 nM), serum withdraw (20 and 5 days), heat shock (43 °C,1 h), Camptothecin (10 μM) and H₂O₂ (100 μM)