Fig. 2

Updated relay board and wireless communication contact closure system (WCCCS) sender board #2, attached to the Agilent 1290 Infinity Flex II autosampler and Universal Interface Box II (UIB II) relays (A-D). The top (smaller) board was a relay board to convert the 5 V TTL signal from the autosampler to a simple contact closure (CC). Relays from the UIB II were simple CCs, so were directly connected to the relay board output (relay A) and WCCCS sender inputs (relays B-D). The switch on the left allowed selecting autosampler or Relay A as the start signal; the selected output is daisy-chained from channels 1 to 13. Relay B was sent to the WCCCS switchable channel 14, Relay C to channel 15, and Relay D to channel 16. All WCCCS signals were switchable between the Agilent 1200 HPLC and the Agilent 1290 Infinity Flex II UHPLC via a switch manifold