Fig. 1

Exposure result of membrane—rs3218550C>T (exposure time: 5 min). Lane 1: probe rs3218550C>T—wild-type allele; Lane 2: probe rs3218550C>T—wild-type allele with specific competition probe (non-biotinylated) and nuclear protein (concentration 1–6 μg/20 μl); Lane 3: probe rs3218550C>T—wild-type allele with nuclear protein (concentration 1—6 μg/20 μl); Lane 4: probe rs3218550C>T—wild-type allele with nuclear protein (concentration 2–12 μg/20 μl); Lane 5: probe rs3218550C>T—variant allele; Lane 6: probe rs3218550C>T—variant allele with specific competition probe (non-biotinylated) and nuclear protein (concentration 1–6 μg/20 μl); Lane 7: probe rs3218550C>T—variant allele with nuclear protein (concentration 1–6 μg/20 μl); Lane 8: probe rs3218550C>T—variant allele with nuclear protein (concentration 2–12 μg/20 μl)