Fig. 1

Details on production of soybean seed and RNAseq analysis of seed germination. a Weather parameters, planting/harvest dates and approximate period of soybean seed fill (stage R5-R8) for genotypes PI 598982A and S99-11986 produced in Columbia, MO (Conventional Soybean Production System) and Stoneville, MS (Early Soybean Production System). b Kinetics of seed germination in this study. Seed of two genotypes, PI 587982A-heat tolerant and S99-11986-heat sensitive, produced in either Columbia, MO (Conventional Soybean Production System) and Stoneville, MS (Early Soybean Production System) were imbibed and germination observed for 72 h (n = 3, 25 seeds per replicated). The mean of three replicates is plotted and error bars indicate standard deviation. c Total genes expressed in each sample with an average FPKM ≥ 0.3. d Distribution of differential gene expression amongst 20 different comparisons. Upregulation indicates higher level of expression in the top sample as compared to the bottom sample. The names indicated in the horizontal axis for c and d are three-letter codes which indicates genotype (P = PI 587982A, S = S99-11986), location seed were produced (C = CSPS, E = ESPS), and tissue (M = dry, mature seed; I = 6-h imbibed seed; G = germinated seed)