Fig. 2

Sensitivity and transcriptional response of hyl1-2 and ire1ab mutants to tunicamycin. a Effect of tunicamycin on survival of wild-type, hyl1-2, and ire1ab. Seeds of wild-type (WT), hyl1-2, and ire1ab were sown on half-strength MS medium with indicated concentrations of tunicamycin and grown for 10 days. The percentage of seeds that survived was calculated. b qRT-PCR analysis of wild-type and hyl1-2 and ire1ab mutant seedlings to tunicamycin. Ten-day-old wild-type and hyl1-2 and ire1ab mutant seedlings were treated with 5 μg/mL tunicamycin for indicated times and subjected to qRT-PCR analysis for detecting BiP3 and At5g40010. The expression values of indicated genes were normalized to that of Act8. c qRT-PCR analysis of wild-type and hyl1-2 mutant seedlings to tunicamycin. Ten-day-old wild-type and hyl1-2 mutant seedlings were treated with 5 μg/mL tunicamycin (+Tm) or 0.1% DMSO (−Tm) as a solvent control for 5 h, and subjected to qRT-PCR analysis for detecting indicated genes. The expression values of indicated genes were normalized to that of Act8. d qRT-PCR analysis of wild-type and hyl1-2, se-1, and ago1-46 mutant seedlings to tunicamycin. Ten-day-old wild-type and mutant seedlings were treated with tunicamycin or DMSO and subjected to qRT-PCR analysis for BiP3 and CRT2 as in c