Table 1 ELISAs inter-assay CV, dilution and detection rates for COBLL1 (Cordon-Bleu Protein-Like 1), CSH1 (Chorionic Somatomammotropin Hormone 1), HSD3B1 (Hydroxy-Delta-5-Steroid Dehydrogenase), EGFL6 (Epidermal Growth Factor-Like Protein 6), XAGE3 (X Antigen Family Member 3), S100P (Calcium Binding Protein P), PAPPA-1 (Pappalysin-1), ERBB2 (Receptor tyrosine kinase 2)
From: Screening circulating proteins to identify biomarkers of fetal macrosomia
Protein | Company | Inter-assay CV or intra? | Dilution | Detection range (pg/ml) |
|---|---|---|---|---|
COBLL1 | myBiosource | Inter-assay < 10% Intra-assay < 10% | Neat | 5000–100,000 |
sCSH1 | myBiosource | Inter-assay CV < 10% Intra-assay CV < 10% | 1:20 | 2500–160,000 |
HSD3B1 | myBiosource | Inter-assay CV < 10% Intra-assay CV < 8% | 1:200 | 313–10,000 |
EGFL6 | myBiosource | Inter-assay CV < 10% Intra-assay CV < 8% | Neat | 78–5000 |
XAGE3 | myBiosource | Inter-assay CV < 15% Intra-assay CV < 15% | Neat | 250–8000 |
S100P | myBiosource | Inter-assay CV < 10% Intra-assay CV < 8% | Neat | 78–5000 |
PAPPA-1 | R&D | N/A | 1:40 | 781–50,000 |
ERBB2 | R&D | N/A | 1:20 | 54.7–3500 |