Skip to main content

Table 1 ELISAs inter-assay CV, dilution and detection rates for COBLL1 (Cordon-Bleu Protein-Like 1), CSH1 (Chorionic Somatomammotropin Hormone 1), HSD3B1 (Hydroxy-Delta-5-Steroid Dehydrogenase), EGFL6 (Epidermal Growth Factor-Like Protein 6), XAGE3 (X Antigen Family Member 3), S100P (Calcium Binding Protein P), PAPPA-1 (Pappalysin-1), ERBB2 (Receptor tyrosine kinase 2)

From: Screening circulating proteins to identify biomarkers of fetal macrosomia

Protein Company Inter-assay CV or intra? Dilution Detection range (pg/ml)
COBLL1 myBiosource Inter-assay < 10%
Intra-assay < 10%
Neat 5000–100,000
sCSH1 myBiosource Inter-assay CV < 10%
Intra-assay CV < 10%
1:20 2500–160,000
HSD3B1 myBiosource Inter-assay CV < 10%
Intra-assay CV < 8%
1:200 313–10,000
EGFL6 myBiosource Inter-assay CV < 10%
Intra-assay CV < 8%
Neat 78–5000
XAGE3 myBiosource Inter-assay CV < 15%
Intra-assay CV < 15%
Neat 250–8000
S100P myBiosource Inter-assay CV < 10%
Intra-assay CV < 8%
Neat 78–5000
PAPPA-1 R&D N/A 1:40 781–50,000
ERBB2 R&D N/A 1:20 54.7–3500