Fig. 2
From: Comparative analysis of IGFBP-3 gene sequence in Egyptian sheep, cattle, and buffalo
a The PCR products of the IGFBP-3 gene from genomic DNA of tested breeds digested by HaeIII. Lanes: 1 and 2; Rahmani, lanes: 3 and 4; Barki, and lanes: 5 and 6; Rahmani X Barki crosses. M, 50 bp DNA ladder, P; PCR product for IGFBP-3. The digestion with HaeIII revealing a single pattern only for 8 DNA fragments sized 201, 201, 87, 67, 57, 18, 16 and 7 bp and indicating absence of a polymorphism in tested sheep breeds for IGFBP-3 gene. The restriction fragments with sizes; 18, 16 and 7 bp were not seen on the gel. b HaeIII restriction pattern of cattle IGFBP-3 gene. Lane M: 100-bp ladder marker. Lanes 1 and 2; homozygous (BB) genotype with 8 restricted fragments at 199, 164, 154, 56, 36, 18, 16 and 8 bp, Lanes 3 and 4; heterozygous (AB) genotype with 5 restricted fragments at 199, 194, 164, 56 and 36 bp and Lanes 5 and 6; homozygous (AA) genotype with 8 restricted fragments at 194, 164, 159, 56, 36, 18, 16 and 8 bp