Fig. 1

Measurement of DNA-DSB by γH2AX fluorescence for the different cell lines following daunorubicin (DNR) treatment. a MOLT-4 cells; b CCRF-CEM cells; c SUP-B15 cells. Cells were exposed to 9 nM NU7026 or 100 nM RI-2 alone or in combination with 10 μM DNR followed by 4 h, 12 h and 24 h in recovery media and the control treatment contained the vehicle dimethyl sulfoxide. Median intensities from histograms of the flow cytometry raw data were used to plot the bar diagrams (mean of total of six replicates ± SD, from three independent experiments). Statistical significance (****P < 0.0001; ns not significant P > 0.05) was determined by two-way ANOVA followed by Tukey’s multi-comparisons test