Table 1 Bacterial HBGA and lectin binding activity with corresponding Western blot band size
From: Characterization of human norovirus binding to gut-associated bacterial ligands
HBGA/Lectin | H/Ulex europaeus (α-l-fucose: H type 2) | AB/Dolichos biflorus (GalNAc: A type 1) | ||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Protein size (kDa) | 140 | 100 | 70 | 50 | 35 | 25 | 17 | 15 | 140 | 100 | 70 | 50 | 35 | 25 | 17 | 15 |
S. aureus | −/+ | +/− | +/− | +/+ | +/− | +/− | +/− | |||||||||
E. cloacae | +/+ | +/− | −/+ | +/+ | −/+ | |||||||||||
Klebsiella spp. | +/+ | −/+ | +/− | +/+ | −/+ | |||||||||||
Bacillus spp. | −/+ | −/+ | +/− | +/+ | +/+ | +/+ | +/− | +/+ | ||||||||
E. faecium | −/+ | +/+ | +/− | −/+ | ||||||||||||
Citrobacter spp. | +/+ | +/+ | −/+ | +/− | +/− | +/+ | −/+ | |||||||||
H. alvei | +/− | +/+ | −/+ | +/− | +/− | +/+ | ||||||||||
HBGA/Lectin | B/Bandeiraea simplicifolia (α -d-Gal: B) | |||||||
|---|---|---|---|---|---|---|---|---|
Protein size (kDa) | 140 | 100 | 70 | 50 | 35 | 25 | 17 | 15 |
S. aureus | +/+ | +/− | +/− | |||||
E. cloacae | +/− | +/− | +/− | −/+ | ||||
Klebsiella spp. | +/− | +/− | −/+ | |||||
Bacillus spp. | +/+ | −/+ | +/− | |||||
E. faecium | +/+ | |||||||
Citrobacter spp. | +/− | +/− | +/− | −/+ | +/− | |||
H. alvei | +/− | +/− | +/+ | +/− | ||||