|
Enzyme assay performance
|
|---|
|
Enzyme
|
Mean V (nmol/s/mg prot)
|
Standard deviation
|
N
|
Intra assay CV (%)
|
|---|
|
Aldolase
|
1.07
|
0.08
|
4
|
7.7
|
|
G6PD
|
0.16
|
0.01
|
4
|
7.2
|
|
PGI
|
12.0
|
1.9
|
3
|
15.7
|
|
PGM
|
0.81
|
0.07
|
3
|
9.2
|
|
Performance of entire experimental procedure
|
|---|
|
Enzyme
|
Mean V (nmol/s/mg prot)
|
Standard deviation
|
N
|
Sample CV (%)
|
|---|
|
Aldolase
|
1.07
|
0.16
|
12
|
16.1
|
|
G6PD
|
0.16
|
0.06
|
11
|
14.6
|
|
PGI
|
12.0
|
2.0
|
9
|
16.4
|
|
PGM
|
0.84
|
0.12
|
9
|
14.8
|
- Aldo Average enzymatic activities of aldolase, G6PD lucose 6 phosphate dehydrogenase, PGI phosphoglucose isomerase and PGM phosphoglucomutase determined in INS-1E cell lysate, and average enzymatic activities of Aldo, G6PD, PGI and PGM determined in biological replicates of INS-1E cell extracts derived from 3 different cell passages
