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Table 1 Enzyme assay performances

From: Physiological phenotyping of mammalian cell lines by enzymatic activity fingerprinting of key carbohydrate metabolic enzymes: a pilot and feasibility study

Enzyme assay performance

Enzyme

Mean V (nmol/s/mg prot)

Standard deviation

N

Intra assay CV (%)

Aldolase

1.07

0.08

4

7.7

G6PD

0.16

0.01

4

7.2

PGI

12.0

1.9

3

15.7

PGM

0.81

0.07

3

9.2

Performance of entire experimental procedure

Enzyme

Mean V (nmol/s/mg prot)

Standard deviation

N

Sample CV (%)

Aldolase

1.07

0.16

12

16.1

G6PD

0.16

0.06

11

14.6

PGI

12.0

2.0

9

16.4

PGM

0.84

0.12

9

14.8

  1. Aldo Average enzymatic activities of aldolase, G6PD lucose 6 phosphate dehydrogenase, PGI phosphoglucose isomerase and PGM phosphoglucomutase determined in INS-1E cell lysate, and average enzymatic activities of Aldo, G6PD, PGI and PGM determined in biological replicates of INS-1E cell extracts derived from 3 different cell passages