Fig. 2

LC-MS/MS analysis of the 23 kDa band identifies 16 mouse proteins. a Migration of proteins immunoprecipitated by anti-C/EBPβ antibody (ab32358) or non-specific rabbit IgG from differentiating C2C12 myoblasts, stained with silver stain. Input is 3% of material used for immunoprecipitation. The rectangle highlights the bands that were subject to MS analysis. b Western blot analysis of the anti-C/EBPβ IP samples performed as in a with a rectangle identifying the unknown band. c Scaffold Viewer data for mouse proteins detected by MS from a. d Myog,Myh8, Myh3, Myl4, Myl1 and Myl12b gene expression in proliferating (GM) primary myoblasts and differentiated for 2 and 5 Days (D2 and D5) obtained from publicly available microarray data (GSE24811) [12]. Data are presented as Log2 to the GM samples. e Western blot analysis of the anti-C/EBPβ IP samples performed as in a and incubated with anti-MYL4 (ab231800)