Fig. 2

A representative case of synergistic interaction of MCT4 and GPC3 in HCC. Photos of GPC3-immunostained section are shown in the upper panel (a and b), and MCT4 immunostaining photos of the serial section are shown in the lower panel (c and d). b and d are magnified images of the dashed square in a and c, respectively. HCC cells were diffusely positive for GPC3 and partly positive for MCT4. The area with stronger GPC3 immunoreactivity is selectively positive for MCT4 (double asterisks) and GPC3-positive portion with weaker immunoreactivity is negative for MCT4 (asterisk). Arrows in b indicate the boundary of the asterisk-marked area and double asterisk-marked area. Scale bars are 1 mm (a and c) and 100 µm (b and d)