Table 1 List of mouse placental proteins whose expression differed significantly between control and Nrk−/− 2DE performed on proteins from E18.5 concepti, as identified by LC-MS/MS analysis
Spot Nr | Protein description | Protein entry | Accession | Score | avgMass | seqCover (%) | Effect sizea | SD | t test (n = 6) |
|---|---|---|---|---|---|---|---|---|---|
1 | Protein 2210010C04Rik OS Mus musculus GN 2210010C04Rik PE 2 SV 1 | Q9CPN9_MOUSE | Q9CPN9 | 435 | 26422 | 8.10 | − 0.710 | 0.653 | 0.009 |
2 | Heat shock protein HSP 90 beta OS Mus musculus GN Hsp90ab1 PE 1 SV 3 | HS90B_MOUSE | P11499 | 102 | 83281 | 3.59 | − 0.248 | 0.126 | 0.020 |
3 | Stress 70 protein mitochondrial OS Mus musculus GN Hspa9 PE 1 SV 3 | GRP75_MOUSE | P38647 | 1030 | 73461 | 39.62 | − 0.018 | 0.774 | 0.942 |
4 | Protein Serpinb9f OS Mus musculus GN Serpinb9f PE 2 SV 1 | Q80UK5_MOUSE | Q80UK5 | 535 | 43034 | 16.98 | − 0.126 | 0.096 | 0.148 |
5 | Annexin A3 OS Mus musculus GN Anxa3 PE 1 SV 4 | ANXA3_MOUSE | O35639 | 556 | 36384 | 27.24 | 0.127 | 0.169 | 0.062 |
6 | Staphylococcal nuclease domain containing protein 1 OS Mus musculus GN Snd1 PE 1 SV 1 | SND1_MOUSE | Q78PY7 | 50 | 102,088 | 4.95 | 0.109 | 0.682 | 0.846 |
7b | – | – | – | – | – | – | − 0.285 | 0.555 | 0.492 |
8 | Protein 2210010C04Rik OS Mus musculus GN 2210010C04Rik PE 2 SV 1 | Q9CPN9_MOUSE | Q9CPN9 | 727 | 26422 | 4.86 | 0.309 | 0.233 | 0.031 |
9 | Pyruvate kinase isozymes M1 M2 OS Mus musculus GN Pkm PE 1 SV 4 | KPYM_MOUSE | P52480 | 3716 | 57845 | 50.66 | − 0.272 | 0.498 | 0.294 |
10b | – | – | – | – | – | – | 0.221 | 0.347 | 0.198 |
11 | Protein disulfide isomerase A5 OS Mus musculus GN Pdia5 PE 2 SV 1 | PDIA5_MOUSE | Q921X9 | 302 | 59267 | 15.67 | 0.666 | 0.816 | 0.116 |
12 | Glutamate dehydrogenase 1 mitochondrial OS Mus musculus GN Glud1 PE 1 SV 1 | DHE3_MOUSE | P26443 | 176 | 61337 | 10.75 | 0.021 | 0.539 | 0.654 |
13 | T complex protein 1 subunit alpha OS Mus musculus GN Tcp1 PE 1 SV 3 | TCPA_MOUSE | P11983 | 465 | 60449 | 30.22 | 0.217 | 0.271 | 0.208 |
14 | 40S ribosomal protein SA OS Mus musculus GN Rpsa PE 1 SV 4 | RSSA_MOUSE | P14206 | 3256 | 32838 | 47.46 | 0.110 | 0.148 | 0.115 |
15 | Annexin A5 OS Mus musculus GN Anxa5 PE 1 SV 1 | ANXA5_MOUSE | P48036 | 12996 | 35753 | 83.39 | − 0.114 | 0.179 | 0.223 |
16 | SPARC OS Mus musculus GN Sparc PE 1 SV 1 | SPRC_MOUSE | P07214 | 126 | 34450 | 11.59 | − 0.256 | 0.597 | 0.235 |
17 | Calpain small subunit 1 OS Mus musculus GN Capns1 PE 2 SV 1 | CPNS1_MOUSE | O88456 | 951 | 28463 | 31.97 | − 0.068 | 0.303 | 0.531 |
18 | Annexin A2 OS Mus musculus GN Anxa2 PE 1 SV 2 | ANXA2_MOUSE | P07356 | 14770 | 38676 | 67.26 | 0.102 | 0.410 | 0.509 |