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Table 1 Richardsonius balteatus (redside shiner) assay specificity with potentially sympatric species

From: Development of a quantitative PCR assay for detection of redside shiner (Richardsonius balteatus) from environmental DNA

Species tested

Common name

DNA amplification success (mean cycle threshold with 10 pg genomic DNA)

Source location

Richardsonius balteatus

Redside shiner

Yes (31.41)

Dragoon Creek, WA; Rock Island bypass, WA; Mercer Creek, WA; Walla Walla River, WA

Pomoxis nigromaculatus

Black crappie

No

Lake Washington, WA

Mylocheilus caurinus

Peamouth

No

Lake Washington, WA

Spirinchus thaleichthys

Longfin smelt

No

Lake Washington, WA

Oncorhynchus nerka

Sockeye salmon

No

Lake Washington, WA

Oncorhynchus tshawytscha

Chinook salmon

No

Lake Washington, WA

Oncorhynchus clarkii

Cutthroat

No

Lake Washington, WA

Ptchocheilus oregonensis

Pikeminnow

No

Lake Washington, WA

Micropterus dolomieu

Smallmouth bass

No

Lake Washington, WA

Perca flavescens

Yellow perch

No

Lake Washington, WA

Ambloplites rupestris

Rock bass

No

Lake Washington, WA

Lepomis macrochirus

Bluegill

No

Lake Washington, WA

Ameiurus nebulosus

Brown bullhead

No

Lake Washington, WA

Oncorhynchus kisutch

Coho salmon

No

Lake Washington, WA

Catostomus macrocheilus

Largescale sucker

No

Lake Washington, WA

Micropterus salmoides

Largemouth bass

No

Lake Washington, WA

Tinca tinca

Tench

No

Lake Washington, WA

Lepomis gibbosus

Pumpkinseed

No

Lake Washington, WA

Cyprinus carpio

Common carp

No

Lake Washington, WA

Gasterosteidae

Stickleback

No

Lake Washington, WA

Alosa sapidissima

Shad

No

Lake Washington, WA

Salvelinus fontinalis

Brook trout

No

Elwha River, WA

Salvelinus confluentus

Bull trout

No

Elwha River, WA

Oncorhynchus mykiss

Rainbow trout

No

Elwha River, WA

Oncorhynchus gorbuscha

Pink salmon

No

Elwha River, WA

Oncorhynchus keta

Chum salmon

No

Elwha River, WA

Gasterosterus aculeatus

Threespine stickleback

No

Big Beef Creek, WA

Thaleichthys pacificus

Eulochon

No

Pacific Ocean off Oregon

Hypomesus pretiosus

Surf smelt

No

Gulf of Alaska, AK

Novumbra hubbsi

Olympic mudminnow

No

Lang Lake, WA

Rhinichthys cataractae

Longnose dace

No

North Fork Asotin Creek, WA