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Table 2 The number of qPCR technical replicates (out of 3 technical replicates performed on each field-collected water sample) that were positive for redside shiner eDNA for each sample site

From: Development of a quantitative PCR assay for detection of redside shiner (Richardsonius balteatus) from environmental DNA

Site (latitude, longitude)

Collection date

Water sample 1 (No. positive)

Water sample 2 (No. positive)

Water sample 3 (No. positive)

Site total

Elwha River estuary, WA (48.14748, −123.56100)

4/22/2015

3/3

1/3

1/3

5/9

Lake Kachess, WA (47.34074, −121.25293)

5/31/2018

3/3

2/3

3/3

8/9

Ross Lake, WAa

8/22/2018

3/3

3/3

6/6

Upper Elwha River, WA (47.81049, −123.45560)

9/21/2017

0/3

0/3

  1. A dashed line (–) indicates a water sample was not collected
  2. aRoss Lake water sample 1 was collected at latitude 48.440728 and longitude −121.034186 and water sample 2 was collected at latitude 48.523052 and longitude −121.010296