Table 2 The number of qPCR technical replicates (out of 3 technical replicates performed on each field-collected water sample) that were positive for redside shiner eDNA for each sample site
Site (latitude, longitude) | Collection date | Water sample 1 (No. positive) | Water sample 2 (No. positive) | Water sample 3 (No. positive) | Site total |
|---|---|---|---|---|---|
Elwha River estuary, WA (48.14748, −123.56100) | 4/22/2015 | 3/3 | 1/3 | 1/3 | 5/9 |
Lake Kachess, WA (47.34074, −121.25293) | 5/31/2018 | 3/3 | 2/3 | 3/3 | 8/9 |
Ross Lake, WAa | 8/22/2018 | 3/3 | 3/3 | – | 6/6 |
Upper Elwha River, WA (47.81049, −123.45560) | 9/21/2017 | 0/3 | – | – | 0/3 |